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Hot Start PCR Reagents and Kits

Hot Start PCR Reagents and Kits

In hot start PCR, key reagents are prevented from reacting in the PCR mixture until optimal temperatures are met. This reduces non-specific DNA amplification that occurs naturally at lower temperatures, increasing product yield, specificity, and sensitivity.
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Detection Method 
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For Use With (Application) 
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1 – 12 of 12 results
1

Invitrogen™ Platinum™ Hot Start PCR Master Mix (2X)

Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 1 X
Overhang 3'-A
Concentration 2X
Polymerase Platinum Taq DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Standard
Label or Dye Unlabeled
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Platinum™
2

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
GREENER_CHOICE

Contains everything required for successful PCR amplification in a convenient package—all components are premixed and premeasured

GC-Rich PCR Performance High
Fidelity (vs. Taq) 1 X
Overhang 3'-A
Green Features Sustainable packaging
Concentration 2X
For Use With (Application) Cloning
Exonuclease Activity 5' - 3'
Detection Method Primer-probe
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
Product Line AmpliTaq Gold™
3

Invitrogen™ Platinum™ II Hot-Start PCR Master Mix (2X)

Invitrogen™ Hot-Start PCR Master Mix for fast and easy PCR

EDGE
GC-Rich PCR Performance High
Format Tube
4

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase
GREENER_CHOICE

Efficiently and reproducibly amplifies long (up to 5 Kb) sequences

Promotions are available
EDGE
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1 X
Overhang 3'-A
Green Features Sustainable packaging
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
Detection Method Primer-probe
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
Product Line AmpliTaq Gold™
5

Invitrogen™ Platinum™ II Hot-Start Green PCR Master Mix (2X)

Invitrogen™ Hot-Start Green PCR Master Mix for fast and easy PCR

GC-Rich PCR Performance High
Format Tube
6

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
7

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

Reaction Format Separate Components
Product Type DNA Polymerase
Overhang 3'-A
Concentration 5 U/μL
Product Line AmpliTaq Gold™
8

Invitrogen™ Platinum™ II Green PCR Buffer (5X)

Invitrogen™ 5X Platinum II Green PCR Buffer for fast and easy PCR

9

Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase

Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

EDGE
Shipping Condition Dry Ice
Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Reaction Format Separate Components
Product Line Platinum™
10

Invitrogen™ Platinum™ Green Hot Start PCR Master Mix (2X)

Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 1 X
Overhang 3'-A
Concentration 2X
Polymerase Platinum Taq DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Standard
Label or Dye Green
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Platinum™
11

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

Promotions are available
Product Type DNA Polymerase
Overhang 3'-A
Concentration 5 U/μL
12

Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix

Premix, hot start chemistry for end-point PCR analysis

EDGE
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1 X
Overhang 3'-A
Concentration 2X
For Use With (Application) Cloning
Exonuclease Activity 5' - 3'
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Size (Final Product) 1.5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Fast PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line AmpliTaq Gold™